Unveiling the ideal conditions for Protein refolding

E Coli, has been a time tested ideal system for expression of heterologous proteins. However production of soluble functional proteins still remains a challenge to date. Oftentimes the over expression of a protein in E.coli results in the formation of insoluble inclusion bodies. The challenge often faced is to recover the protein from these insoluble particles. The identification of the conditions needed to properly refold the protein remains empirical.

Employing a fractional factorial matrix design, QuickFold™ Protein Refolding Screening Kit allows a quick screen for wide range of buffer conditions in one single experiment. It contains 15 different buffer compositions for rapid identification of the factors effecting protein refolding. This allows the researcher to rapidly pinpoint the optimal buffer formulation for any protein. For this and many other reagents for protein expression, purification and recovery, please visit our website or call us  today to place a quick order.